Natural products are important leads in drug discovery, represent a reservoir of diverse templates and are being tapped to outsource novel anticancer agents many other plants have been examined to identify new and effective anti cancer compounds. The purpose of present study was designed to investigate cytotoxic activity of hydromethanolic leaf extract of syzygiumjambos (L) Alston, in-vitro cytotoxic evaluation was determined by using MTT assay on MCF-7 (Breast Cancer); HT-29 (Human colorectal cancer) A-549 cell lines (Human lung cancer) and Vero cell lines. To determine cytotoxic activity various concentration of plant extract were used. Test drug was separately dissolved in distilled DMSO and volume was made up with DMEM supplemented with 2% inactivated FBS to obtain a stock solution of 1 mg/ml concentration and sterilized by filtration. Serial two fold dilutions were prepared from this for carrying out cytotoxic studies. By using ELISA reader wavelength measured at 540 nm and percentage of cell inhibition was determined by plotting a graph of concentration vs. percentage of inhibition. The concentration of plant extract required to inhibit 50 % of cell growth (IC 50) values was determined.
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